Sciences Biologiques

URI permanent de cette collectionhttps://dspacee.univ-temouchent.edu.dz/handle/123456789/753

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Contribution à une étude épidémiologique de la fièvre boutonneuse dans la wilaya d’Ain Témouchent
(2026) BENHADDOU Karima Samia; BENHOURA Loubna; BENSAAD Fatima Zahra; MOGHTIT Fatima Zohra
Mediterranean spotted fever (MSF), a zoonotic disease transmitted primarily through the bite of the brown dog tick (Rhipicephalus sanguineus), continues to pose a significant public health challenge in Algeria. The aim of this study was to contribute to characterize the regional epidemiology of MSF in the Wilaya of Aïn Témouchent and to perform a detailed assessment of the epidemiological, clinical, and hospital-related features of reported cases. This retrospective descriptive study was conducted using two complementary datasets. The first comprised 1,128 cases of Mediterranean spotted fever reported by the various Primary Public Healthcare Facilities in the Wilaya of Aïn Témouchent between January 2021 and December 2025. The second involved a detailed analysis of the clinical and laboratory characteristics of 50 patients hospitalized at Ahmed Medaghri Hospital in Aïn Témouchent. The epidemiological analysis demonstrated a substantial increase in MSF incidence in 2023, with 427 reported cases. Women represented 58% of affected individuals, and most patients were from rural settings (82%). A clear seasonal distribution was observed, with 69% of infections occurring during the summer period. Epidemiological investigations identified tick bites (84%) and contact with dogs (78%) as the principal exposure factors. Among hospitalized patients, the clinical picture was characterized by the typical triad of fever, maculopapular rash, and an inoculation eschar (tache noire). The most frequent laboratory abnormalities were thrombocytopenia, leukopenia or leukocytosis, and increased liver transaminase levels consistent with hepatic cytolysis. Most patients responded well to doxycycline therapy and experienced favorable outcomes, although a limited number developed severe disease with multiorgan involvement. In conclusion, the diagnosis of MSF remains primarily based on clinical findings and epidemiological history. Early administration of appropriate antibiotic therapy is associated with a favorable outcome and complete recovery in the vast majority of patients.
Analyse statistique du profil des femmes atteintes du syndrome des ovaires poly kystique
(2026) KEDADRA Douaa; KHERAFA Ahlem; MEDJAOUI Ikram
Polycystic Ovary Syndrome (PCOS) is one of the most common endocrine disorders affecting women of reproductive age. It is associated with various clinical, hormonal, and metabolic abnormalities that may impair reproductive health. This study aimed to describe the clinical, hormonal, and metabolic characteristics of 30 women diagnosed with PCOS from the provinces of Oran and Ain Témouchent, Algeria. The results showed a mean age of 28.33 ± 5.89 years and a mean body mass index (BMI) of 27.41 ± 5.71 kg/m2, indicating a tendency toward overweight. Menstrual cycle disorders were highly prevalent (93.33%), with dysmenorrhea, amenorrhea, and oligomenorrhea being the most frequently reported abnormalities. Hirsutism was the most common clinical manifestation (53.33%), followed by acne (46.67%) and alopecia (30%). Biological assessment revealed a mean LH/FSH ratio of 2.02 ± 0.79 and a mean HOMA-IR index of 3.31 ± 1.39, suggesting the presence of insulin resistance in a proportion of patients. Phenotype A was the most frequent phenotype (36.67%). No statistically significant differences were observed between PCOS phenotypes for the studied parameters (P > 0.05). These findings highlight the clinical, hormonal, and metabolic heterogeneity of PCOS and highlight the importance of early diagnosis and appropriate management to prevent reproductive and metabolic complications associated with this syndrome.
Évaluation de la qualité bactériologique et organoleptique des Saucisses type « Merguez » commercialisées au niveau de la ville d’Ain Témouchent.
(2026) DALILE DJAOUAHIR; ATTAR FATIMA ZOHRA; AHMED AMMAR YAMINA
"Merguez" is among the meat products most exposed to microbiological alterations and risk of contamination by microorganisms of hygienic and sanitary concern. This study mainly aims to evaluate the bacteriological quality of merguez-type sausages commercialized in the city of Aïn Témouchent. An organoleptic evaluation of the samples was also performed to assess their sensory characteristics and correlate them with the bacteriological results. In total, 05 samples were collected from different retail outlets across the city (local butcher shops). The microbiological analysis focused on the enumeration of hygiene indicator and pathogenic microorganisms, notably Total Mesophilic Aerobic Flora (FMAT) and fecal coliforms, as well as the detection of specific pathogens such as Staphylococcus aureus and Salmonella. In parallel, the organoleptic evaluation was based on the criteria of color, odor, appearance, and texture. The microbiological results reveal a worrying situation with a variable overall quality ranging between satisfactory (20%), acceptable (40%), and unsatisfactory (40%), reflecting deficiencies during the grinding, stuffing, or cold chain maintenance stages, or a high initial microbial load of the raw material. On the organoleptic level, most samples exhibited an acceptable visual quality despite a high underlying microbial contamination, thereby highlighting that organoleptic evaluation alone is insufficient to conclude on the hygienic quality of the product. It must therefore be interpreted in conjunction with microbiological and physicochemical analyses. This study emphasizes the imperative need to strengthen hygiene measures and intensify official controls.
Mise en évidence des spores de champignons mycorhiziens dans les sols salins de la région de Terga, wilaya d'Aïn Témouchent.
(2026) SAADALLAH Abir; BOUAZZA-MAROUF Khadidja; BOUCHOUICHA Sara
Soil salinization is one of the major environmental constraints affecting natural ecosystems and agricultural production worldwide. This phenomenon leads to the deterioration of soil physicochemical and biological properties, resulting in reduced plant growth and productivity. In this context, arbuscular mycorrhizal fungi (AMF) represent a promising biological strategy due to their ability to enhance nutrient uptake, water acquisition, and plant tolerance to abiotic stresses, particularly salinity. The present study was conducted in the Terga region (Aïn Témouchent Province, Algeria) and aimed to characterize the physicochemical properties of the soil and to assess its mycorrhizal potential through the detection of arbuscular mycorrhizal fungi associated with Salicornia spp. . Physicochemical analyses revealed an alkaline soil pH ranging from 8.27 to 8.83 and an electrical conductivity varying between 2.68 and 3.78 dS m−1, indicating moderate saline conditions. Microscopic examination of stained roots confirmed the presence of characteristic mycorrhizal structures, demonstrating root colonization by AMF. Spore extraction and observation revealed an active mycorrhizal potential, with a higher spore density in rhizospheric soil compared to bare soil. The highest spore density reached 131 spores in rhizospheric soil versus 52 spores in bare soil. The results indicate that the saline soils of Terga harbor active mycorrhizal communities capable of establishing symbiotic associations with Salicornia spp. despite harsh environmental conditions. This symbiosis may contribute significantly to the adaptation of halophytic plants to saline environments and could represent a valuable biological resource for the restoration and sustainable management of degraded saline soils.
Évolution spatiale et temporelle de la brucellose humaine dans la région d Ain-Temouchent
(2026) BENZOURA Douaa Meriem; BOUSSAID Ikram Kheira; BOUAMRA Mohammed
Brucellosis is a zoonotic disease caused by the bacterium Brucella, with a global incidence estimated at 500,000 human cases per year. Despite efforts made in terms of health policy in Algeria, it remains a major concern in the country. Our study aims to determine the spatial and temporal evolution of human brucellosis in the Ain-Temouchent region from 2014 to 2025. This was a retrospective study, and the data were collected from the wilaya's public health directorate. Based on the results obtained, 907 cases of human brucellosis were recorded, with an average annual rate of 75.58 cases, representing an average annual incidence of 17.5 cases per 100,000 inhabitants. A clear predominance of male cases was observed throughout the study period (60.74% in men versus 39.25% in women). All age groups were affected, with a higher prevalence among those aged 55 and over. The results show that human brucellosis can occur in any season and month of the year, but it is more frequent in spring and summer. These results demonstrate that brucellosis remains a significant problem in the Ain Témouchent province. To improve the current situation, efforts to combat brucellosis must be intensified to control the spread of the disease among at-risk groups and reduce its incidence.
Etude de la situation actuelle de la rage dans la wilaya de Ain Temouchent
(2026) HAMIANI Amel; KADDACHE Ghofrane; MADANI Khadidja
Rabies is a fatal viral disease that affects all mammals, including humans. However, it can be prevented through vaccination and prompt, appropriate post-exposuretreatment. This study focuses on the epidemiology of human and animal rabies in the wilaya of Ain Témouchent, during the period 2021–2025. Data were collected from the Directorate of Health and Population (DSP) and the Veterinary Inspection Service of the Directorate of Agricultural Services (DSA), and were analyzed using Microsoft Excel. The results showed a progressive increase in the number of people exposed to animal bites during the study period, with a total of 13214 cases recorded between 2021 and 2025. Rabies can affect all age groups; however, adults aged 15 years and over were the most affected, with a predominance of males. Despite the increase in exposure cases, no human rabies cases were reported during the study period, thanks to effective post-exposure prophylaxis and adequate vaccination coverage. Regarding animal rabies, dogs accounted for the majority of confirmed cases. Cats and donkeys represented a smaller number of cases, while no cases were reported in cattle. In addition, animal vaccination campaigns resulted in the vaccination of a large number of animals. The results of this study highlight the need to strengthen dog vaccination, improve the control of stray animals, and intensify public awareness campaigns in order to sustainably reduce the risk of transmission of this zoonotic disease.
Profil biochimique et hématologique des femmes enceintes diabétiques de la population d’Aïn Témouchent
(2026) BERRIAH Abir; BERRAMDANE Hada Dounia; KHOLKHAL Fatima
Gestational diabetes mellitus represents a major public health concern due to its consequences on maternal and fetal health. This condition is associated with various metabolic and hematological disturbances that may influence the course of pregnancy and promote the occurrence of maternal and fetal complications. The objective of this work is to evaluate certain biochemical and hematological parameters in pregnant women affected by gestational diabetes mellitus. The studied parameters notably include blood glucose, glycated hemoglobin (HbA1c), lipid profile, as well as several hematological parameters. This study was carried out on 60 pregnant women divided into two groups: a group of 30 women with gestational diabetes mellitus and a control group composed of 30 healthy pregnant women. The analysis of the results revealed differences between the two groups regarding several of the biological parameters studied. The obtained results show that diabetic pregnant women present higher mean values of blood glucose and HbA1c than those observed in healthy pregnant women. Variations in certain hematological parameters were also observed, whereas several biochemical parameters remained within the reference values. These findings highlight the importance of regular biochemical and hematological monitoring in pregnant women with gestational diabetes mellitus in order to ensure better pregnancy surveillance and contribute to the prevention of maternal and fetal complications.
Prévalence et impact du marqueur CA15-3 dans le pronostic du cancer du sein
(2026) Belouadi Ikhlas Rofaida; Belkhedim Wissem; TAHARI F Z
Breast cancer is one of the most common cancers among women worldwide. Breast cancer is characterised by the uncontrolled proliferation of breast cells, primarily affecting the milk ducts or lobules, and has the ability to spread to the opposite breast as well as to other distant organs, forming metastases. Its development is associated with several risk factors: non- modifiable (age, family history, BRCA genetic mutations) and modifiable (obesity, alcohol consumption, smoking, age at first pregnancy). The classification of breast cancer is based on molecular subtypes (luminal A, luminal B, HER2-positive, triple-negative), histopathological types (in situ and invasive) and TNM staging. Our retrospective study, conducted among 18 patients aged 20 to 80 years, identified the main epidemiological, clinical and biological characteristics. The results show a predominance of patients aged 50 to 60 and who were married (89.36%).Clinically, frequent lymph node involvement was observed, with tumours predominantly affecting the left breast (57.14% for CA 15-3 < 30 U/ml), particularly in the upper outer quadrant (OUQ) with 60.71% for CA 15-3 < 30 U/ml and 45.45% for CA 15-3 > 30 U/ml. Histologically, grade II SBR invasive ductal carcinoma (IDC) was the most common. Strong expression of hormone receptors (ER+/PR+) was noted, associated with elevated CA15-3 levels, particularly in HER2-positive patients. These results confirm the value of CA15-3 for post-treatment monitoring.
Recherche d'activités biologiques dans les graines de Cucurbita pepo
(2026) HICHOUR Halima; ABDELMALEK Nesrine; BENYAMINA Sofiane Mourad
The seeds of Cucurbita pepo serve as plant storage structures that function as a genuine natural bioactive complex. These seeds are distinguished by a rich chemical composition, that confers several biological properties, notably antioxidant, enzymatic, anti-inflammatory, and antimicrobial activities. In this study, two biological activities (antioxidant and enzymatic activities) were investigated within aqueous solution of Cucurbita pepo seeds. Antioxidant activities were evaluated by monitoring the degradation of H2O2 (catalase activity) and by the DPPH radical-scavenging assay, whereas amylase and phytase activities were assessed, respectively, by determining the amount of degraded starch and the amount of released inorganic phosphate as a function of pH, temperature, and salinity. The results showed the presence of a catalase activity (complete degradation of the initial H2O2 concentration, 5 mM) and a DPPH radical-scavenging activity with an IC50 value of 8.36 mg/mL. Furthermore, the aqueous extract of these seeds exhibited, after 5 min of incubation at 30 °C, an amylase activity (degradation of 18.18 μg starch/mg protein) and a phytase activity (release of 0.6 mM phosphate/mg protein), with optima at 30 °C and pH 9 for amylases, and 50 °C and pH 5 for phytases. In the presence of salt, both enzymes were affected, with phytases being more sensitive. Stability studies showed that amylases retained little activity after thermal treatment (about 9%) and were sensitive to both acidic and alkaline media (25% and 31.81% residual activity, respectively), while remaining highly stable in saline medium (72.72%). Conversely, phytases withstand thermal treatment (25% residual activity) and acidic pH (55%), but completely lost their activity under alkaline and saline conditions. The results obtained confirm that Cucurbita pepo seeds can constitute an exploitable source of antioxidant and enzymatic activities that could be valorized in industrial and biotechnological sectors.
Impact d’un mode de vie sain chez les patientes atteintes d’un cancer du sein. Étude clinique. Service d’oncologie. Hôpital Dr Benzerdjeb
(2026) BENDRISS Bouchra; BENGRISSA Latéfa; M ABI-AYAD
Breast cancer is the most frequently diagnosed malignancy in women worldwide, representing a major public health challenge. In this context, the adoption of healthy lifestyle measures – commonly referred to as a 'healthy lifestyle' – is attracting increasing interest in oncology due to its potential effects on biological biomarkers, clinical status, and patients' quality of life. This interventional study was conducted on 10 patients with confirmed breast cancer monitored at the oncology department of Hôpital Dr Benzerdjeb, Aïn Témouchent (21/03/2026– 10/06/2026). For one month, participants followed a protocol combining daily walking (≥30 min), vitamin D supplementation (200,000 IU), a diet low in refined sugars, twice-weekly intermittent fasting, and sleep hygiene measures. Results showed a notable increase in vitamin D levels (28.27 → 39.61 ng/mL), improved nutritional status (albumin: 33.20 → 41.96 g/L), and reduced systemic inflammation (CRP: 7.65 → <6 mg/L). Clinically, asthenia decreased significantly (50% → 12.5%). However, none of these changes reached statistical significance (p >0.05), likely due to the small sample size and short intervention period. These encouraging findings support further studies with larger cohorts and longer follow-up to better quantify the real-world benefits of lifestyle interventions in integrative breast cancer care.
Evaluation du potentiel antimicrobien des actinomycètes extrémophiles de la région d’Ain Témouchent vis-à-vis des micro-organismes responsables d’in- fection urinaire
(2026) MAKDAD Rim; MADANI Atika; BENBELAID Fethi
Urinary tract infections, already the most common infectious diseases worldwide, are exacer- bated by the increasing emergence of bacterial strains resistant to conventional antibiotics. Among the solutions explored, we focused on studying extremophile Actinomycetes isolated from three sites in the Aïn Témouchent region (the Hassi El Ghella salt flat, the Ourdania Cave, and the Sidi Aïd mineral spring) with the aim of finding alternative antimicrobial molecules. Characterization of the strains using phenotypic tests, based on macroscopic and microscopic features, as well as physiological and biochemical tests, allowed us to classify them within the genus Streptomyces. The evaluation of antimicrobial activity by the agar cylinder method demonstrated that the isolated strains exert significant inhibition on several bacteria responsible for urinary tract infections, including Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Enterococcus faecalis, as well as on yeasts of the genus Candida.
Supplémentation en vitamine D3, chrome et régime sans sucre chez les patients diabétiques : EPSP el amria
(2026) Hadjoudja Kheira; Meryem ABI-AYAD
This work aims to evaluate the impact of a combined vitamin D3 and chromium supplementation in patients with type 2 diabetes. The study consisted of assessing and comparing two key metabolic biomarkers — fasting glucose and glycated hemoglobin (HbA1c) — before and after the supplementation period. The results revealed an improvement in glycemic parameters among the monitored patients. Indeed, the mean fasting glucose decreased from 1.37±0.53 g/L (among 14 patients evaluated before the intervention) to 1.14 ± 0.35 g/L (among 10 patients evaluated after the supplementation). Furthermore, a notable reduction in the mean HbA1c was recorded, dropping from 7.17± 1.71 % to 6.33 ± 1.44 %. These data suggest a favorable and beneficial effect of vitamin D3 and chromium supplementation on glycemic control in type 2 diabetic patients, although postsupplementation HbA1c values remain close to the prediabetes zone according to the ADA criteria.
Evaluation des propriétés technologiques de souches de lactobacillus pour starters laitiers
(2026) BENDAHMANE Fatima Zohra; BENEDDIF Fatima Zohra; DAHMANE Hana; DJOUAD Mokhtar Eddine
A total of 46 strains related to the genus Lactobacillus sensu lato were isolated and characterized based on their morphological, physiological, and biochemical properties. The strains S3, S13, S17, and S27 were identified using the API 50 CHL system as Lactobacillus brevis, Lactobacillus curvatus, Lactobacillus plantarum, and Lactobacillus paracasei subsp. paracasei, respectively, revealing an interesting microbiological diversity within the studied samples. The evaluation of technological properties showed that the strains exhibited good tolerance to NaCl concentrations of 2% and 4%, as well as a predominant production of β-galactosidase. Strains S23, S2, S3, and S4 demonstrated significant proteolytic activity, while positive lipolytic activity was observed in approximately 55% of the tested strains. In contrast, no exopolysaccharide production was detected. The assessment of functional properties revealed variable resistance to acidic conditions (pH 2 and pH 3) and bile salts, with strains S31 and S34 showing the highest tolerance. Furthermore, the strains exhibited stronger antimicrobial activity against Staphylococcus aureus than against Escherichia coli, indicating their ability to inhibit certain undesirable microorganisms. These results highlight the microbiological richness of local raw milk and emphasize the potential interest of certain strains for applications in the dairy industry. However, further studies are needed to deepen the characterization of these isolates and confirm their potential for practical use.
Etude de la croissance des moisissures isolées à partir du sol salin de Sebkha d’Oran : Region de Misserghin
(2026) FATMI Amel Yasmine; BENCHIHA Meriem Amira; ZIANE Mohammed
Saline soils constitute extreme environments characterized by high salt concentrations and specific physicochemical conditions. Despite these constraints, they harbor a microbial diversity capable of adapting to osmotic stress. The present study aimed to investigate the fungal diversity of saline soils and to evaluate the growth capacity of fungal isolates under different temperature conditions and NaCl concentrations. Molds were isolated on PDA and Czapek Dox Agar media supplemented with 10% and 15% NaCl and incubated at 25°C through direct cultivation of soil fragments from four soil samples. The fungal isolates were identified based on microscopic observations. The identification results revealed the presence of three fungal genera: Penicillium spp, Fusarium spp, and Aspergillus spp. The growth study of these isolates at different temperatures and NaCl concentrations showed that growth depended on the fungal isolate. However, regardless of the NaCl concentration, 25°C was identified as the optimal temperature for growth. In conclusion, this study highlights the fungal diversity present in the saline soils of the Oran Sebkha and contributes to a better understanding of fungal biodiversity in Algerian hypersaline environments.
Recherche de germes pathogènes majeurs responsables des infections mammaires chez la vache laitière dans la région d’Ain Témouchent
(2026) MAHIMDA Ahlem; BOUAMRA Mohammed
Mastitis remains one of the three major challenges to global dairy production, resulting in significant economic losses. Current estimates of the specific prevalence of each pathogen are essential for effective disease surveillance and control. The objective of this study was to contribute to the identification of major pathogens responsible for mastitis infections in dairy cows in the Ain Témouchent region. We screened 170 lactating cows using the Californian Mastitis Test (CMT). Milk samples that tested positive for CMT underwent bacteriological analysis. Our results show that mastitis remains a dominant disease in cattle farming. Indeed, we recorded a prevalence of 44.11% of subclinical mastitis cases. Based on the results of bacteriological analyses, a predominance was recorded for coagulase-negative Staphylococcus (45.51%), followed by Staphylococcus aureus (22.75%) and Streptococcus spp. (14.25%). Other isolated bacterial species included Escherichia coli (9.20%), Klebsiella pneumoniae (3.57%), and Pseudomonas spp. (1.36%). Antibiotic susceptibility testing revealed the existence of resistance, with varying proportions depending on the antibiotic class. In conclusion, it is better to take precautions to improve production and maintain good health than to have to treat an illness.
Contribution à l’étude du potentiel mycorhizogène des sols salins de la région du Douaima (Ain Temouchent)
(2026) Hadjoub Chaimaa; Kadri Nor El Houda Manel; Bouazza Marouf Khadidja
The main objective of this study is to evaluate the mycorrhizogenic potential of saline soils in the Douaima wetland, in the commune of Hammam Bouhajar, Aïn Témouchent province. To achieve this, several physico-chemical and biological indicators were studied : soil texture, pH, electrical conductivity, redox potential, total dissolved solids (TDS), NaCl content, organic matter content, and calcium carbonate dosage. The selected biological indicators are root mycorrhizal colonization, spore density, and mycorrhizal infective potential (MIP). Particular attention was given to the impact of the pioneer halophyte species Salicornia fruticosa on the mycorrhizal dynamics of the rhizospheric soils. The results revealed a relatively low spore density across all soils (238 spores/g in soil 1 and 199 spores/g in soil 2, compared to 182 spores/g in bare soil), probably due to salinity constraints. The mycorrhizal infective potential (MIP) was moderate in the rhizospheric soils (42.13 for soil 1 and 32.40 for soil 2) and very low in bare soil (66.89). On the physico-chemical level, the soils have a silty- clayey texture (soil 1 and bare soil) to silty-sandy (soil 2), a moderate organic matter content (2.04% in soil 1, 4.22% in soil 2, and 2.57% in bare soil), an alkaline pH (7.95 in soil 1, 8.12 in soil 2, and 8.11 in bare soil), and moderate to average electrical conductivity. The obtained results show that, despite the constraints imposed by salinity, the rhizospheric soils associated with Salicornia fruticosa maintain a functional mycorrhizal potential. These observations suggest that this halophyte could contribute to the maintenance of arbuscular mycorrhizal fungi communities in saline environments. Thus, AMF could constitute an interesting lever for restoration and rehabilitation programs of saline soils in coastal wetlands.
Évaluation de l’effet d’amendements organiques issus de sous-produits agroalimentaires sur la fertilité des sols et la croissance d’une culture test
(2026) Benzina nariman; Bentouir mama; Bourtal zerat Hanane; YAZIT SIDI-MOHAMMED
The valorization of agro-food by-products and their use as organic soil amendments represent a sustainable approach that contributes to improving soil fertility and reducing organic waste. In this context, this study aims to evaluate the effect of two types of organic amendments, namely eggshell and wheat bran, on some physicochemical properties of the soil as well as on the growth of a test crop of lettuce (Lactuca sativa L.). The experiment was conducted under controlled conditions using four treatments: control soil (T0), soil amended with eggshell (T1), wheat bran (T2), and a mixture of eggshell and wheat bran (T3). Several soil properties, including pH, electrical conductivity, and organic matter content, were analyzed before and after cultivation in addition to monitoring lettuce growth parameters. The results showed that the addition of organic amendments influenced soil properties and plant development. Eggshell application contributed to increasing soil pH and maintaining values close to neutrality due to its high calcium carbonate content, whereas wheat bran improved soil organic matter content and enhanced lettuce growth. The different treatments showed variations in soil and plant responses, highlighting the agronomic potential of these agro-food by-products as organic amendments. In conclusion, the use of eggshell and wheat bran as organic soil amendments represents a promising ecological and economic alternative for improving soil fertility and supporting plant production.
Etude ethnobotanique des plantes médicinales utilisées dans le traitement traditionnel des cancers
(2026) Guedoudou Feriel; Mekhanter Meriem; Tahari Fatima Zohra
Cancer is one of the most concerning diseases. In some regions, people traditionally use medicinal plants to try to treat it. This study focuses on the medicinal plants used to treat cancer in the Ain Temouchent region. The objective is to inventory, document, and analyze the medicinal plants used by the local population in traditional cancer treatment. The survey was conducted from February to March 2026 with 30 informants, including herbalists and elderly women possessing ethnomedical knowledge. Data collection was based on a semi-structured questionnaire administered face-to-face. The data were analyzed using descriptive statistical analysis.The results identified 36 plant species. The five most frequently mentioned plants were Nigella sativa (black seed), Thymus vulgaris (thyme), and Artemisia spp. (wormwood), Curcuma longa (turmeric), and Atriplex halimus (orache). The most frequently mentioned types of cancer are breast cancer (52.63%) and colon cancer (31.58%). The dominant preparation methods are powder, infusion, and decoction, administered primarily orally. This study constitutes an original scientific contribution to the documentation of the ethnobotanical heritage of the Ain Temouchent region. It reveals the richness of local traditional knowledge and underscores the urgent need for its preservation in the face of contemporary cultural erosion.
Contribution à l’étude des souches de bactéries lactiques isolées à partir du lait de chèvre
(2026) Boudissa Ainouna Mounia; Abdelhakem Khouloud; Daghbadje Warda; BELLAHCENE Mouloud
This study focused on the isolation and characterization of lactic acid bacteria from raw goat milk collected in the Ain Témouchent region. The experimental work involved physicochemical analyses, including the measurement of pH and titratable acidity. Several microbiological analyses were also carried out to investigate the presence of total flora, coliforms, staphylococci, yeasts and molds, as well as lactic acid bacteria in goat milk samples. After isolation and purification, the obtained isolates were subjected to various identification tests, including Gram staining, catalase and oxidase tests, growth at different temperatures, pH levels, and NaCl concentrations, determination of the fermentative type, thermoresistance testing, and growth on Sherman milk-based medium. The antagonistic activity of the isolates against three pathogenic microorganisms was also evaluated, and an antibiotic susceptibility test was performed. The results revealed the presence of Gram-positive and catalase-negative lactic acid bacteria, showing good adaptation to various growth conditions and inhibitory activity against certain pathogenic microorganisms. This study therefore confirms the importance of raw goat milk as a source of lactic acid bacteria that can be exploited in the agri-food and biotechnological fields.
Étude de l’activité antimicrobienne de trois plantes médicinales de la région d’Aïn Témouchent : Artemisia herba-alba, Thymus coloratus et Jasonia tuberosa
(2026) BENZINA Zohra; BERRADJA Narimene; BENBELAID Fethi
The increasing resistance of microorganisms to antibiotics has become a major public health concern, highlighting the need to explore new natural antimicrobial alternatives. The aim of this study was to evaluate the antimicrobial activity of the essential oils of Artemisia herba-alba, Thymus coloratus, and Jasonia tuberosa against several bacterial and fungal strains.The essential oils were extracted by hydro distillation. Their antimicrobial activity was assessed using the disk diffusion method (aromatogram), as well as by determining the Minimum Inhibitory Concentrations (MICs) and the Minimum Bactericidal/Fungicidal Concentrations (MBCs/MFCs).The results showed that the essential oil of Artemisia herba-alba exhibited the strongest antimicrobial activity, followed by Thymus coloratus, whereas Jasonia tuberosa demonstrated weak activity. These findings highlight the promising potential of essential oils, particularly that of Artemisia herba-alba, as a natural source of antimicrobial agents that may contribute to combating microbial resistance.

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