BOUKADA, Fatna AchganeFEDDANE, BouchraY. AHMED, AMMAR OUADAH2025-07-142025-07-142025http://dspace.univ-temouchent.edu.dz/handle/123456789/6668Antibiotic resistance, which is constantly evolving, affects all classes of antibiotics. This retrospective study, conducted from January to February 2025 in private laboratories in Aïn Témouchent, assessed the resistance of Enterobacteriaceae isolated from various clinical samples (urine, stool, pus, and genital secretions). The results revealed a predominance of urinary isolates (67%), particularly among women (60%), with Escherichia coli being the most frequently isolated species (49%). High resistance rates were observed, especially to doxycycline (85.71%), ampicillin (55%), and trimethoprim-sulfamethoxazole (48%). Klebsiella isolates showed the most concerning resistance levels, ranging from 40% to 100%. Enteric (stool) isolates also displayed alarming resistance rates (30% to 81%). Multidrug resistance was significantly present: 77% of isolates were resistant to at least three antibiotics, 50% to five, and 11% to more than seven. This situation threatens the effectiveness of current treatments and highlights the urgent need for rational antibiotic use.frAntibiotic resistance – Antibiotics – Enterobacteriaceae – Multidrug resistanceThesis