Synthesis of silver nanoparticles using saussurea costus and study of its antimicrobial and antioxidant properties

Abstract

Recently, silver nanoparticles have gained attention because of their antimicrobial activity which offers the possibility of their use for medical purposes. Metallic nanoparticles can be obtained by physical, chemical or biological methods. Therefore, in the present study, an attempt was made to formulate a cost effective and environment friendly technique for green synthesis of silver nanoparticles. The silver nanoparticles were synthesized using extract of Costus specious with AgNO3 solution. Saussurea Costus is a plant medicinal used in traditional medicine. The as-formed AgNPs were analyzed by (UV-Vis), FT-IR and X-ray diffraction (XRD) . The synthesis of AgNPs was confirmed by the characteristic color change, yellow to brown and was observed under UV–Vis Spectroscopy monitored at 435 nm. Additionally, in the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical antioxidant study, the antimicrobial properties against Gram-positive, Gram-negative pathogens and fungal strain using method of diffusion of antibiotic disks on Muller Hinton (MH) agar of the obtained silver nanoparticles at different concentrations were investigated. The results showed that These FTIR and DRX methods confirmed the presence and molecular interaction of silver nanoparticles and their crystalline nature. AgNPs presented the highest anti-radical activity with 50 μg/ml IC50 followed by ethanolic extract with (IC50 30 μg/ml). the antimicrobial study showed that the bacteria had a high sensibility against the Ag NPs formed and against the extract especially candida albicans, 18 mm for the AgNPs and 12mm for the Costus Extract. This green chemistry is very advantageous for AgNPs biosynthesis, for example, cost-effectiveness and usability for medicinal, pharmaceutical and extensive industrial applications. Furthermore, the bio-recovery unit for plant extracts provides a greater ease of handling, compared to micro-organisms