Optimisation des méthodes d’extraction et caractérisation de la phycocyanine extraite à partir de la spiruline Algérienne et sa concervation

Abstract

Spirulina.platensis, more commonly called Spirulina, is a blue-green algae by the presence of chlorophyll (green) and phycocyanin (blue) contained in phycobilisomes. Our work focuced first of all one the caracterisationofSpirulinastrains Algeria ,(region of bnisaf).then, we evaluated several methods of exraction for obtained of a good mass of thephycocyanin Six methods were tested on theSpirulinastrains considerd: by water, freezing, solvent, sonification,double phase aqueous separation and maceration with glycerol showed that the extraction by double phase aqueous separation gave a good concentration (1.8mg / ml) The second part of our work focused on the comparison of the extraction yield of the westernstrain while preserving on the one hand, its intrinsic qualities, and on the other hand, to be able to deduce afterwards, the optimal method in terms of yield Finally, our study allowed us to compare between the methods of extraction of phycocyanin from Spirulinaproduced in western Algeria with a view to optimization.