Activité anti-oxydante et anti-microbienne de l’huile essentielle d’une plante médicinale Ammoides verticillata de la région d’Ain temouchent

Abstract

The work of this Master aimed to study the antibacterial and antioxidant activity of the essential oil of an aromatic medicinal plant: Ammoides verticillata, the extraction of essential oils was carried out by hydrodistillation. The antioxidant activity of the oil studied showed very promising antioxidant effect by DPPH methods. A. verticillata EO showed a very high antioxidant capacity with IC50s of 0.125 mg/ml. The antioxidant capacity of A. verticillata EO was higher than that of the ascorbic acid and BHT controls. The antimicrobial activity of essential oils was studied against nine bacterial strains: three Gram-negative Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Salmonella typhi (ATCC 14028), six Gram-positive : Staphylococcus aureus (ATCC 43300), Clostridium sporogenes (ATCC 19404), Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 7314), Lactobacillus rhamnosus (ATCC 53103) and Bacillus cereus (ATCC14579).Ammoides verticilata EO showed very good inhibitory activity against all the strains tested, with activity ranging from sensitive to extremely sensitive. We also note that this oil was extremely sensitive against both Gram(+) and Gram(-) bacteria.